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Myocytic androgen receptor overexpression does not affect sex differences in adaptation to chronic endurance exercise

To study the dynamics of insulin release, L-arginine (Merck, 1 g/kg body weight dissolved in 0.9% NaCl) was injected ip in animals fasted for 14 h to depolarize the -cell [40, 90] and thus elicit the secretion of insulin from the readily releasable pool (RRP) of insulin granules

This is another reason to use prefilled syringes within 1-2 weeks rather than pushing the full 28-day window